{"id":3879,"date":"2020-11-22T16:32:12","date_gmt":"2020-11-22T09:32:12","guid":{"rendered":"https:\/\/hoatainguyenmoitruong.mitc.edu.vn\/?p=3879"},"modified":"2021-03-29T09:23:19","modified_gmt":"2021-03-29T02:23:19","slug":"di%cc%a3nh-luong-cu2-trong-ma%cc%83u-nuoc-bang-phuong-phap-uv-vis","status":"publish","type":"post","link":"https:\/\/hoatainguyenmoitruong.mitc.edu.vn\/khoa-hoc-cong-nghe\/di%cc%a3nh-luong-cu2-trong-ma%cc%83u-nuoc-bang-phuong-phap-uv-vis.html","title":{"rendered":"\u0110\u1ecbnh l\u01b0\u1ee3ng Cu2+ trong m\u1eabu n\u01b0\u1edbc b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p UV-VIS"},"content":{"rendered":"

\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0ThS. L\u01b0\u01a1ng C\u00f4ng Quang<\/em><\/strong><\/p>\n

\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 Khoa C\u00f4ng ngh\u00ea\u0323 Ho\u0301a-Ta\u0300i nguy\u00ean va\u0300 M\u00f4i tr\u01b0\u01a1\u0300ng<\/em><\/strong><\/p>\n

    \n
  1. \u0110\u0103\u0323t v\u00e2\u0301n \u0111\u00ea\u0300<\/strong><\/li>\n<\/ol>\n

    \u0110\u1ed3ng (Cu)<\/strong>\u00a0l\u00e0\u00a0kho\u00e1ng ch\u1ea5t\u00a0\u0111\u01b0\u1ee3c c\u01a1 th\u1ec3 h\u1ea5p th\u1ee5 \u1edf d\u1ea1 d\u00e0y v\u00e0 ph\u1ea7n tr\u00ean c\u1ee7a ru\u1ed9t non, sau \u0111\u00f3 \u0111i v\u00e0o m\u00e1u.\u00a0\u0110\u1ed3ng\u00a0c\u00f3 t\u00e1c d\u1ee5ngch\u1ed1ng oxy h\u00f3a, ng\u1eeba ung th\u01b0, t\u1ed1t cho tim m\u1ea1ch v\u00e0 r\u1ea5t c\u1ea7n thi\u1ebft cho s\u1ef1 ho\u1ea1t \u0111\u1ed9ng c\u1ee7a h\u1ec7 th\u1ea7n kinh.<\/strong>\u00a0Ch\u1ea5t n\u00e0y c\u00f3 nhi\u1ec1u trong\u00a0rau c\u00f3 m\u00e0u xanh \u0111\u1eadm, khoai t\u00e2y, n\u1ea5m,\u00a0t\u00f4m, cua hay l\u00faa m\u1ea1ch,…\u0110\u1ed3ng \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn nhi\u1ec1u qua\u0301 tr\u00ecnh sinh h\u00f3a c\u1ee7a c\u00e2y nh\u01b0 qu\u00e1 tr\u00ecnh c\u1ed1 \u0111\u1ecbnh N, s\u1ef1 kh\u1eed nitrat, s\u1ef1 kh\u1eed CO2<\/sub>, s\u1ef1 t\u1ed5ng h\u1ee3p chloroplyll, t\u1ed5ng h\u1ee3p c\u00e1c ch\u1ea5t \u0111i\u1ec1u h\u00f2a sinh tr\u01b0\u1edfng, s\u1ef1 tho\u00e1t h\u01a1i n\u01b0\u1edbc, s\u1ef1 chuy\u1ec3n h\u00f3a gluxit, t\u1ea1o c\u00e1c m\u00f4 m\u1edbi th\u00e2n l\u00e1 r\u1ec5, v\u00e0 \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn t\u00ednh\u00a0ch\u1ecbu h\u1ea1n, ch\u1ecbu l\u1ea1nh, ch\u1ecbu n\u00f3ng\u00a0c\u1ee7a c\u00e2y.<\/p>\n

    \u0110\u1ed3ng la\u0300 nguy\u00ean t\u00f4\u0301 vi l\u01b0\u01a1\u0323ng giu\u0301p c\u00e2y tr\u00f4\u0300ng sinh tr\u01b0\u01a1\u0309ng va\u0300 pha\u0301t tri\u00ea\u0309n, \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn s\u1ef1 t\u1ed5ng h\u1ee3p nhi\u1ec1u lo\u1ea1i ch\u1ea5t \u0111\u01b0\u1eddng, b\u1ed9t, h\u1ee3p ch\u1ea5t c\u00f3 \u0111\u1ea1m, ch\u1ea5t b\u00e9o, chloroplyll v\u00e0 c\u00e1c s\u1eafc t\u1ed1 kh\u00e1c, vitamin C v\u00e0 c\u00e1c enzim.<\/p>\n

    \u0110\u1ed3ng t\u1ed3n t\u1ea1i trong n\u01b0\u1edbc d\u01b0\u1edbi d\u1ea1ng ion Cu2+<\/sup>, ho\u1eb7c d\u01b0\u1edbi d\u1ea1ng ph\u1ee9c xianua, tactrat\u2026v\u01a1\u0301i h\u00e0m l\u01b0\u1ee3ng kh\u00f4ng l\u1edbn dao \u0111\u1ed9ng trong kho\u1ea3ng t\u1eeb 0.001 \u0111\u1ebfn 1mg\/l. \u0110\u1ec3 x\u00e1c \u0111\u1ecbnh h\u00e0m l\u01b0\u1ee3ng \u0111\u1ed3ng, ng\u01b0\u01a1\u0300i ta th\u01b0\u01a1\u0300ng s\u01b0\u0309 du\u0323ng ph\u01b0\u01a1ng pha\u0301p AAS, tuy nhi\u00ean \u01a1\u0309 m\u00f4\u0323t s\u00f4\u0301 pho\u0300ng thi\u0301 nghi\u00ea\u0323p ch\u01b0a co\u0301 ma\u0301y AAS co\u0301 th\u00ea\u0309 du\u0300ng ph\u01b0\u01a1ng ph\u00e1p tr\u1eafc quang v\u1edbi thu\u00f4\u0301c th\u01b0\u0309 dietyldithiocarbamate (DDC).<\/p>\n

      \n
    1. Nguy\u00ean l\u00fd cu\u0309a ph\u01b0\u01a1ng pha\u0301p<\/strong><\/li>\n<\/ol>\n

      Ph\u1ea3n \u1ee9ng gi\u1eefa Cu2+<\/sup> v\u1edbi Pb-DDC x\u1ea3y ra t\u1ea1i gi\u00e1 tr\u1ecb pH = 1.0 \u0111\u1ebfn 1.5, trong tr\u01b0\u1eddng h\u1ee3p n\u00e0y ngo\u00e0i \u0111\u1ed3ng c\u00f2n c\u00f3 Bi, Hg, Ag c\u00f9ng tham gia ph\u1ea3n \u1ee9ng, c\u00e1c nguy\u00ean t\u1ed1 c\u00f2n l\u1ea1i kh\u00f4ng tham gia ph\u1ea3n \u1ee9ng n\u00ean ph\u01b0\u01a1ng ph\u00e1p n\u00e0y c\u00f3 \u0111\u1ed9 ch\u1ecdn l\u1ecdc cao v\u00ec Ag v\u00e0 Hg kh\u00f4ng g\u00e2y c\u1ea3n tr\u1edf do ph\u1ee9c c\u1ee7a ch\u00fang kh\u00f4ng m\u00e0u.<\/p>\n

      Lo\u1ea1i b\u1ecf c\u00e1c \u1ea3nh h\u01b0\u1edfng:<\/em><\/strong><\/p>\n

      – Bi: N\u1ebfu h\u00e0m l\u01b0\u1ee3ng n\u00e0y v\u01b0\u1ee3t qu\u00e1 30 \u00b5g\/l th\u00ec c\u1ea7n ph\u1ea3i l\u1eafc ph\u1ee9c DDC kim lo\u1ea1i v\u1edbi 25ml HCl 5M trong 5 ph\u00fat, do \u0111\u00f3 ph\u1ee9c Bi-DDC b\u1ecb ph\u00e2n h\u1ee7y c\u00f2n ph\u1ee9c Cu-DDC kh\u00f4ng b\u1ecb ph\u00e2n h\u1ee7y.<\/p>\n

      – Ph\u1ee9c Cu-xianua: th\u00eam 0,5ml H2\u00ad<\/sub>SO4<\/sub>(1:1), 5ml HNO3<\/sub> \u0111\u1eb7c v\u00e0o 200 \u2013 250ml m\u1eabu ch\u1ee9a trong c\u1ed1c ch\u1ecbu nhi\u1ec7t, l\u00e0m bay h\u01a1i dung d\u1ecbch \u0111\u1ebfn g\u1ea7n c\u1ea1n kh\u00f4 (qu\u00e1 tr\u00ecnh ph\u00e2n h\u1ee7y n\u00ean \u0111\u1eb7t trong t\u1ee7 h\u00fat). \u0110\u1ec3 ngu\u1ed9i, th\u00eam v\u00e0o 1ml HCl \u0111\u1eb7c v\u00e0 l\u00e0m bay h\u01a1i \u0111\u1ebfn kh\u00f4, \u0111\u1ec3 ngu\u1ed9i, th\u00eam n\u01b0\u1edbc c\u1ea5t hai l\u1ea7n, \u0111un n\u00f3ng dung d\u1ecbch \u0111\u1ebfn s\u00f4i \u0111\u1ec3 h\u00f2a tan h\u1ebft l\u01b0\u1ee3ng mu\u1ed1i r\u1eafn, l\u1ecdc v\u00e0 chuy\u1ec3n to\u00e0n b\u1ed9 dung d\u1ecbch v\u00e0o b\u00ecnh\u00a0 m\u1ee9c th\u00edch h\u1ee3p.<\/p>\n

        \n
      1. Thi\u1ebft b\u1ecb v\u00e0 d\u1ee5ng c\u1ee5 va\u0300 ho\u0301a ch\u00e2\u0301t<\/strong><\/li>\n<\/ol>\n

        – Ph\u1ec3u chi\u1ebft th\u1ec3 t\u00edch 250 ml ho\u1eb7c 500ml; m\u00e1y UV-VIS; c\u00e1c d\u1ee5ng c\u1ee5 th\u00f4ng th\u01b0\u1eddng \u1edf ph\u00f2ng h\u00f3a nghi\u1ec7m;<\/p>\n

        – H2<\/sub>SO4<\/sub> 1:1, HNO3<\/sub> \u0111\u1eadm \u0111\u1eb7c, HCl \u0111\u1eadm \u0111\u1eb7c, dung m\u00f4i h\u1eefu c\u01a1 toluene ho\u1eb7c t\u01b0\u01a1ng \u0111\u01b0\u01a1ng, Na-DDC, Pb-DDC trong toluene;<\/p>\n

        Chu\u1ea9n b\u1ecb m\u1ed9t ph\u1ec3u chi\u1ebft s\u1ea1ch th\u1ec3 t\u00edch 1000ml, th\u00eam v\u00e0o 50-100ml n\u01b0\u1edbc c\u1ea5t hai l\u1ea7n, 0.1 g Pb(NO3<\/sub>)2<\/sub> lo\u1ea1i tinh khi\u1ebft ph\u00e2n ti\u0301ch, l\u1eafc k\u0129 \u0111\u1ec3 mu\u1ed1i tan h\u1ebft. H\u00f2a tan 0.1 g Na-DDC trong l\u01b0\u1ee3ng n\u01b0\u1edbc t\u1ed1i thi\u1ec3u r\u1ed3i th\u00eam v\u00e0o ph\u1ec3u chi\u1ebft \u0111\u1ec3 k\u1ebft t\u1ee7a h\u1ebft Pb-DDC, th\u00eam v\u00e0o ph\u1ec3u chi\u1ebft 250ml toluene, \u0111\u1eady n\u00fat ph\u1ec3u chi\u1ebft v\u00e0 l\u1eafc m\u1ea1nh, to\u00e0n b\u1ed9 k\u1ebft t\u1ee7a Pb-DDC s\u1ebd tan h\u1ebft v\u00e0o trong toluene, t\u00e1ch b\u1ecf t\u01b0\u1edbng n\u01b0\u1edbc, ph\u1ea7n t\u01b0\u1edbng h\u1eefu c\u01a1 \u0111\u01b0\u1ee3c l\u1ecdc qua gi\u1ea5y l\u1ecdc cho v\u00e0o b\u00ecnh m\u00e0u n\u00e2u, dung d\u1ecbch b\u1ec1n trong ba th\u00e1ng.<\/p>\n

        – Dung d\u1ecbch Cu2+<\/sup> chu\u1ea9n n\u1ed3ng \u0111\u1ed9 1\u00b5g\/ml.<\/p>\n

          \n
        1. Tr\u00ecnh t\u1ef1 ph\u00e2n t\u00edch<\/strong><\/li>\n<\/ol>\n

          – L\u1ea5y m\u1ed9t th\u1ec3 t\u00edch n\u01b0\u1edbc c\u1ea7n ph\u00e2n t\u00edch sao cho ch\u1ee9a kho\u1ea3ng 0,2 \u2013 0,6 \u00b5gCu\/l, cho v\u00e0o ph\u1ec5u chi\u1ebft co\u0301 th\u1ec3 t\u00edch 200-500ml, pha lo\u00e3ng m\u1eabu n\u01b0\u1edbc n\u1ebfu c\u1ea7n \u0111\u1ebfn th\u1ec3 t\u00edch 100ml r\u1ed3i th\u00eam v\u00e0o 5 gi\u1ecdt HCl(1:1), t\u1eeb buret th\u00eam v\u00e0o m\u1ed9t c\u00e1ch ch\u00ednh x\u00e1c 10ml Pb-DDC trong toluene ho\u1eb7c dung m\u00f4i h\u1eefu c\u01a1 (c\u00f3 th\u1ec3 d\u00f9ng petroleum thay th\u1ebf). C\u1ea9n th\u1eadn \u0111\u1eady n\u00fat ph\u1ec3u chi\u1ebft l\u1ea1i v\u00e0 l\u1eafc trong hai ph\u00fat, gi\u1eef y\u00ean ph\u1ec5u chi\u1ebft \u0111\u1ec3 cho hai t\u01b0\u1edbng ph\u00e2n l\u1edbp, t\u00e1ch b\u1ecf ph\u1ea7n t\u01b0\u1edbng n\u01b0\u1edbc ph\u00eda d\u01b0\u1edbi, ph\u1ea7n t\u01b0\u1edbng h\u1eefu c\u01a1 c\u00f3 ch\u1ee9a Cu-DDC c\u00f3 m\u00e0u v\u00e0ng \u0111\u01b0\u1ee3c chuy\u1ec3n v\u00e0o cuvet v\u00e0 ti\u1ebfn h\u00e0nh so m\u00e0u t\u1ea1i b\u01b0\u1edbc s\u00f3ng 430nm.<\/p>\n

          – L\u1ea5y v\u00e0o c\u00e1c ph\u1ec5u chi\u1ebft: 0.2ml; 0.5ml; 1.0ml; 2.0ml; 3.0ml; 4.0ml; 5.0ml; 6.0ml dung d\u1ecbch chu\u1ea9n Cu2+<\/sup> c\u00f3 n\u1ed3ng \u0111\u1ed9 1\u00b5g Cu\/ml, pha lo\u00e3ng b\u1eb1ng n\u01b0\u1edbc c\u1ea5t \u0111\u1ebfn 100ml v\u00e0 ti\u1ebfn h\u00e0nh chi\u1ebft t\u00e1ch nh\u01b0 m\u1eabu th\u1eed. Ti\u1ebfn h\u00e0nh v\u1edbi m\u1eabu tr\u1eafng song song.<\/p>\n

            \n
          1. T\u00ednh k\u1ebft qu\u1ea3<\/strong><\/li>\n<\/ol>\n

            D\u00f9ng ph\u1ea7n m\u1ec1m \u0111\u1ec3 x\u00e2y d\u1ef1ng \u0111\u01b0\u1eddng chu\u1ea9n. N\u1ebfu R2<\/sup> <0,99 th\u00ec ph\u1ea3i ti\u1ebfn h\u00e0nh l\u1ea1i th\u00ed nghi\u1ec7m. Ng\u01b0\u1ee3c l\u1ea1i t\u00ecm ph\u01b0\u01a1ng tr\u00ecnh \u0111\u01b0\u1eddng chu\u1ea9n y = a.x +b<\/p>\n

            T\u1eeb ph\u01b0\u01a1ng tr\u00ecnh \u0111\u01b0\u1eddng chu\u1ea9n t\u00ednh k\u1ebft qu\u1ea3 ph\u00e2n t\u00edch theo c\u00f4ng th\u1ee9c sau:<\/p>\n

            Trong \u0111\u00f3: y l\u00e0 m\u1eadt \u0111\u1ed9 quang c\u1ee7a m\u1eabu ph\u00e2n t\u00edch<\/p>\n

            a v\u00e0 b l\u00e0 c\u00e1c h\u1eb1ng s\u1ed1<\/p>\n

            V1<\/sub> l\u00e0 th\u1ec3 t\u00edch b\u00ecnh \u0111\u1ecbnh m\u1ee9c (mL);<\/p>\n

            V2<\/sub> l\u00e0 th\u1ec3 t\u00edch m\u1eabu (mL).<\/p>\n

            T\u00c0I LI\u1ec6U THAM KH\u1ea2O:<\/strong><\/p>\n[1]. L\u00ea \u0110\u1ee9c, Gia\u0301o tri\u0300nh<\/em> M\u1ed9t s\u1ed1 ph\u01b0\u01a1ng ph\u00e1p ph\u00e2n t\u00edch m\u00f4i tr\u01b0\u1eddng<\/em>, NXB \u0110\u1ea1i h\u1ecdc Qu\u1ed1c Gia H\u00e0 N\u1ed9i, 2015.<\/p>\n[2]. TCVN 6193:1996 \u2013ISO 8288: 1986(E).<\/p>\n[3]. Vo\u0303 Anh Khu\u00ea, Gia\u0301o tri\u0300nh ca\u0301c ph\u01b0\u01a1ng pha\u0301p ph\u00e2n ti\u0301ch ho\u0301a ly\u0301<\/em>, tr\u01b0\u01a1\u0300ng Cao \u0111\u0103\u0309ng C\u00f4ng th\u01b0\u01a1ng mi\u00ea\u0300n Trung, 2017.<\/p>\n

             <\/p>\n","protected":false},"excerpt":{"rendered":"

            \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0ThS. L\u01b0\u01a1ng C\u00f4ng Quang \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0<\/p>\n","protected":false},"author":14,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[101],"tags":[],"yst_prominent_words":[],"_links":{"self":[{"href":"https:\/\/hoatainguyenmoitruong.mitc.edu.vn\/rest\/wp\/v2\/posts\/3879"}],"collection":[{"href":"https:\/\/hoatainguyenmoitruong.mitc.edu.vn\/rest\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/hoatainguyenmoitruong.mitc.edu.vn\/rest\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/hoatainguyenmoitruong.mitc.edu.vn\/rest\/wp\/v2\/users\/14"}],"replies":[{"embeddable":true,"href":"https:\/\/hoatainguyenmoitruong.mitc.edu.vn\/rest\/wp\/v2\/comments?post=3879"}],"version-history":[{"count":0,"href":"https:\/\/hoatainguyenmoitruong.mitc.edu.vn\/rest\/wp\/v2\/posts\/3879\/revisions"}],"wp:attachment":[{"href":"https:\/\/hoatainguyenmoitruong.mitc.edu.vn\/rest\/wp\/v2\/media?parent=3879"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/hoatainguyenmoitruong.mitc.edu.vn\/rest\/wp\/v2\/categories?post=3879"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/hoatainguyenmoitruong.mitc.edu.vn\/rest\/wp\/v2\/tags?post=3879"},{"taxonomy":"yst_prominent_words","embeddable":true,"href":"https:\/\/hoatainguyenmoitruong.mitc.edu.vn\/rest\/wp\/v2\/yst_prominent_words?post=3879"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}